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p ulk1 ser 555 d1h4 rabbit mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p ulk1 ser 555 d1h4 rabbit mab
    P Ulk1 Ser 555 D1h4 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+ulk1/pmc13068069-6-0-8
    Average 86 stars, based on 1 article reviews
    p ulk1 ser 555 d1h4 rabbit mab - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: Epigenetic regulation of serine biosynthesis by PHF8 during neurogenesis.
    Article Snippet: Antibodies used were anti: PHF8 (Abcam, ab36068), H4K20me1 (Abcam, ab9051), H3K9me2 (Abcam, ab1220), H3K9me3 (Abcam, ab8898), NFIA (Abcam, ab 228897), ULK1 (Cell Signaling, #8054), p-ULK1 (ser 757) (Cell Signaling, #6888), alpha TUBULIN (Abcam, ab4074), p-H2A.X (Ser139) clone JBW301 12 EMBO reports © The Author(s) (Merck, 05-636), IdU (BD biosciences, Cat n° 34758), CldU (Abcam, ab6326), LC3 (MBL, PM036), SQSTM1 (Progen, GP62C), S6K (Cell Signaling, #9202S), p-S6K (thr389) (Cell Signaling, #9234), B-actin (Merck, A2228), TFEB (ProteinTech, 13372-1-AP), GFP (AvesLabs GFP-1010), SOX2 (R&D Systems AF2088), TBR2 (Abcam 183991), TUJ1 (Biolegend 801202), DAPI (Thermo Fisher, D1306), and DCX (Millipore, 324385) was used at 1 μg/ml.

    Article Title: Epigenetic regulation of serine biosynthesis by PHF8 during neurogenesis
    Article Snippet: Antibodies used were anti: PHF8 (Abcam, ab36068), H4K20me1 (Abcam, ab9051), H3K9me2 (Abcam, ab1220), H3K9me3 (Abcam, ab8898), NFIA (Abcam, ab 228897), ULK1 (Cell Signaling, #8054), p-ULK1 (ser 757) (Cell Signaling, #6888), alpha TUBULIN (Abcam, ab4074), p-H2A.X (Ser139) clone JBW301 (Merck, 05-636), IdU (BD biosciences, Cat n° 34758), CldU (Abcam, ab6326), LC3 (MBL, PM036), SQSTM1 (Progen, GP62-C), S6K (Cell Signaling, #9202S), p-S6K (thr389) (Cell Signaling, #9234), B-actin (Merck, A2228), TFEB (ProteinTech, 13372-1-AP), GFP (AvesLabs GFP-1010), SOX2 (R&D Systems AF2088), TBR2 (Abcam 183991), TUJ1 (Biolegend 801202), DAPI (Thermo Fisher, D1306), and DCX (Millipore, 324385) was used at 1 μg/ml.

    Article Title: A Novel Autophagy Inhibitor p -Hydroxylcinnamaldehyde Suppresses Esophageal Squamous Cell Carcinoma by Targeting LDHA Phosphorylation-Mediated Metabolic Reprogramming
    Article Snippet: Antibodies against cleaved caspase-3 (#9664), caspase-3 (#9662), cleaved caspase-8 (#9496), caspase-8 (#4790), cleaved caspase-9 (#9505), caspase-9 (#9508), p-AMPK (#2535), and p-ULK1 (#14202) were sourced from Cell Signaling Technology (Beverly, MA, USA).

    Electrophoresis:

    Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts
    Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (Sigma-Aldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); PGC1 alpha Rabbit Ab (1:1000; Novus Biologicals, NBP1-04676); β-Actin Mouse Ab (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-47778)]; and secondary antibodies [anti-rabbit IgG (goat anti-rabbit, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA sc-2004) or anti-mouse IgG (goat anti-mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:2000; sc-2005)] for the immunodetection of the protein of interest. .. The Invitrogen iBright 1500 Imaging System (Thermo Fisher Scientific, Mississauga, ON, Canada) and the iBright Analysis Software (v. 5.5.0) were used for the semi-quantification of the band intensity.

    Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts.
    Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (SigmaAldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); PGC1 alpha Rabbit Ab (1:1000; Novus Biologicals, NBP1-04676); β-Actin Mouse Ab (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-47778)]; and secondary antibodies [anti-rabbit IgG (goat anti-rabbit, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA sc-2004) or anti-mouse IgG (goat anti-mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:2000; sc-2005)] for the immunodetection of the protein of interest. .. The Invitrogen iBright 1500 Imaging System (Thermo Fisher Scientific, Mississauga, ON, Canada) and the iBright Analysis Software (v. 5.5.0) were used for the semi-quantification of the band intensity.

    Membrane:

    Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts
    Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (Sigma-Aldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); PGC1 alpha Rabbit Ab (1:1000; Novus Biologicals, NBP1-04676); β-Actin Mouse Ab (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-47778)]; and secondary antibodies [anti-rabbit IgG (goat anti-rabbit, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA sc-2004) or anti-mouse IgG (goat anti-mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:2000; sc-2005)] for the immunodetection of the protein of interest. .. The Invitrogen iBright 1500 Imaging System (Thermo Fisher Scientific, Mississauga, ON, Canada) and the iBright Analysis Software (v. 5.5.0) were used for the semi-quantification of the band intensity.

    Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts.
    Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (SigmaAldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); PGC1 alpha Rabbit Ab (1:1000; Novus Biologicals, NBP1-04676); β-Actin Mouse Ab (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-47778)]; and secondary antibodies [anti-rabbit IgG (goat anti-rabbit, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA sc-2004) or anti-mouse IgG (goat anti-mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:2000; sc-2005)] for the immunodetection of the protein of interest. .. The Invitrogen iBright 1500 Imaging System (Thermo Fisher Scientific, Mississauga, ON, Canada) and the iBright Analysis Software (v. 5.5.0) were used for the semi-quantification of the band intensity.

    Immunodetection:

    Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts
    Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (Sigma-Aldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); PGC1 alpha Rabbit Ab (1:1000; Novus Biologicals, NBP1-04676); β-Actin Mouse Ab (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-47778)]; and secondary antibodies [anti-rabbit IgG (goat anti-rabbit, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA sc-2004) or anti-mouse IgG (goat anti-mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:2000; sc-2005)] for the immunodetection of the protein of interest. .. The Invitrogen iBright 1500 Imaging System (Thermo Fisher Scientific, Mississauga, ON, Canada) and the iBright Analysis Software (v. 5.5.0) were used for the semi-quantification of the band intensity.

    Article Title: The Effect of Mechanical Loading on Mitophagy in Aged Myoblasts.
    Article Snippet: Total protein extracts were collected from C2C12 myoblasts using RIPA Buffer (SigmaAldrich, St.Louis, MO, USA, R0278-50ML) mixed with phosphatase inhibitor (Cell Signaling Technology, Danvers, MA, USA, #5870S) and protease inhibitor (Cell Signaling Technology, #5871S) Cocktail. .. The extracts underwent electrophoresis (SDS-PAGE), were transferred to a polyvinylidene fluoride (PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000; Cell Signaling Technology, #12396), Parkin Mouse Ab (1:1000; Cell Signaling Technology, #4211); PGC1 alpha Rabbit Ab (1:1000; Novus Biologicals, NBP1-04676); β-Actin Mouse Ab (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-47778)]; and secondary antibodies [anti-rabbit IgG (goat anti-rabbit, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA sc-2004) or anti-mouse IgG (goat anti-mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:2000; sc-2005)] for the immunodetection of the protein of interest. .. The Invitrogen iBright 1500 Imaging System (Thermo Fisher Scientific, Mississauga, ON, Canada) and the iBright Analysis Software (v. 5.5.0) were used for the semi-quantification of the band intensity.

    Western Blot:

    Article Title: VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy
    Article Snippet: .. The primary antibodies used in western blots were as follows: β-actin (1:1000, T002; Affinity, Cincinnati, OH, USA); GAPDH (1:5000, 104941-AP; Proteintech, Wuhan, China); P-gp (1:3000, 22336-1-AP; Proteintech); CD9 (1:1000, sc-13118; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), HSP90 (1:5000, 13171-1-AP; Proteintech); calnexin (1:1000; c-23954; Santa Cruz Biotechnology, Inc. USA); TSG101 (1:1000, c-7964; Santa Cruz Biotechnology, Inc.); VDAC1 (1:1000, PTM-6157; PTM Biolabs, Chicago, IL, USA); p62 (1:1000, #5114; Cell Signaling Technology, Danvers, Massachusetts, USA); LC3B (1:1000, L7543; Sigma); ULK1 (1:1000, 20986-1-AP; Proteintech); p-ULK1 (1:1000, #14202; Cell Signaling Technology); AMPK (1:1000, #5831; Cell Signaling Technology); p-AMPK (1:1000, #2535; Cell Signaling Technology); mTOR (1:5000, 20657-1-AP; Proteintech); p-mTOR (1:2000, 67778-1-Ig; Proteintech); p70S6K (1:2000, 14485-1-AP; Proteintech); p-p70S6K (1:1000, #AF3228; Affinity); ATG5 (1:1000, 10181-2-AP; Proteintech); ATG7 (1:1000, 10088-2-AP; Proteintech); HTRA1 (1:1000, 55011-1-AP, Proteintech); and STEAP4 (1:1000, 11944-1-AP; Proteintech). .. Cells pretreated under specified conditions were harvested and plated in 96-well plates (Corning, NY, USA) at a density of 4000 cells per well.



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